De adem van de aarde CO2 ontsnapt uit bodem

[Henk Elegeert]

REPLY TO: D66 at nic.surfnet.nl

Henk Elegeert wrote:

> REPLY TO: D66 at nic.surfnet.nl

http://www.onderzoekinformatie.nl/nl/oi/nod/onderzoek/OND1305509/
Project: Activiteit, snelheden, verbruik van koolstof en hoge-druk
microbiële ecologie van de diepzee (ARCHIMEDES) (www.onderzoekinformatie.nl)

"
Activity, Rates, Carbon use and HIgh-pressure Microbial Ecology of the
DEep Sea (ARCHIMEDES)

Samenvatting-Eng:
    	We propose to study the activity of the prokaryotic community in
the two largest but least-known oceanic subsystems, the meso-
(150-1000m) and bathypelagic (1000-4000m) realm. Our knowledge on the
phylogenetic composition of the prokaryotic communities in the dark
ocean has substantially advanced over the past decade. Their actual
metabolic activities, however, still remain essentially unknown and can
only be deduced at the moment from model predictions in combination with
a few actual rate measurements under in situ pressure. These model
predictions and the few data available indicate that prokaryotic
communities in these dark realms of the ocean are more active than
commonly measured under decompressed conditions. We propose to directly
measure prokaryotic activity under in situ pressure conditions by
combining conventional methods to determine the metabolic activity of
the bulk prokaryotic (Bacteria and Archaea) community with recent
methodological developments on single cell approaches in the deep ocean.
In situ pressure-retaining systems will be used to assess, for the first
time ever, cell production, respiration and ectoenzymatic hydrolytic
activity of the bulk prokaryotic community and compare these rates with
those on decompressed samples. By combining the recently developed
catalyzed reporter deposition fluorescence in situ hydridization
(CARD-FISH) with microautoradiography (MICRO-CARD-FISH) we will
determine the uptake of specific organic compounds by selected Bacteria
and the Cren- and Euryarchaea in the meso- and bathypelagic realm and
assess their population/group-specific pressure sensitivity.
Additionally, the diversity of the prokaryotic ectoenzymatic hydrolases
playing a key role in the turnover of DOM (glucosidases, proteases,
phosphatases) will be determined by capillary electrophoretic separation
of the ectoenzymes. The 2 newly developed methods MICRO-CARD-FISH and
the capillary electrophoretic separation of ectoenzymes have been
previously tested during the TRANSAT cruises. Both methods offer
unsurpassed sensitivity and resolution. Thus, we have the tools
available now to determine actual metabolic rates of prokaryotes in the
meso- and bathypelagic realm.

Looptijd  	12/2004 - 12/2007
...
"

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